Cap-independent co-expression of dsRNA-sensing and NF-κB pathway inhibitors enables tunable self-amplifying RNA expression with reduced immunotoxicity
Tony K.Y. Lim, Anne Ritoux, Luke W Paine, Larissa Ferguson, Tawab Abdul, Laura J Grundy, Ewan St. John Smith
This paper develops a strategy to reduce immunotoxicity in self-amplifying RNA by encoding immune suppressors that bypass translation shutdown, enabling sustained transgene expression in mouse cells.
eLife · Aug 29, 2025Peer-reviewedBioengineering