A large-scale whole-genome sequencing analysis reveals highly specific genome editing by both Cas9 and Cpf1 nucleases in rice
Xu Dong Tang, Guanqing Liu, Jianping Zhou, Qiurong Ren, Qi You, Li Tian, Xuhui Xin, Zhaohui Zhong, Binglin Liu, Xuelian Zheng, Dengwei Zhang, Aimee A. Malzahn, Zhiyun Gong, Yiping Qi, Tao Zhang, Yong Zhang
This study uses whole-genome sequencing to analyze off-target mutations in Cas9 and Cpf1-edited rice, finding most mutations arise from tissue culture rather than nuclease activity.
Genome biology · Jul 4, 2018Peer-reviewedMolecular Biology