Genome-wide analyses of PAM-relaxed Cas9 genome editors reveal substantial off-target effects by ABE8e in rice
Yuechao Wu, Qiurong Ren, Zhaohui Zhong, Guanqing Liu, Yangshuo Han, Yu Bao, Li Liu, Shuyue Xiang, Shuo Liu, Xu Dong Tang, Jianping Zhou, Xuelian Zheng, Simon Sretenovic, Tao Zhang, Yiping Qi, Yong Zhang
The paper analyzes off-target effects of PAM-relaxed Cas9 editors in rice using whole-genome sequencing and finds ABE8e causes ~500 A-to-G mutations per plant at TA motifs.
Plant Biotechnology Journal · May 7, 2022Peer-reviewedGenetics