Tao Zhang

miR-6818-5p Drives Ovarian Granulosa Cell Dysfunction in PCOS via Targeting HSD17B2 and Modulating PI3K/Caspase-9 Axis

High performance TadA-8e derived cytosine and dual base editors with undetectable off-target effects in plants

This study develops and evaluates TadA-8e-derived cytosine and dual base editors in plants, showing high efficiency, purity, and specificity with no off-target effects.

Genome-wide investigation of multiplexed CRISPR-Cas12a-mediated editing in rice

The paper investigates off-target effects of multiplexed CRISPR-Cas12a editing in rice using whole-genome sequencing and finds low-frequency chromosomal deletions and duplications with high DSB numbers.

Genome-wide analyses of PAM-relaxed Cas9 genome editors reveal substantial off-target effects by ABE8e in rice

The paper analyzes off-target effects of PAM-relaxed Cas9 editors in rice using whole-genome sequencing and finds ABE8e causes ~500 A-to-G mutations per plant at TA motifs.

A universal chromosome identification system for maize and wild Zea species

The paper develops a universal chromosome identification system for maize and wild Zea species using FISH probes, enabling accurate identification and revealing chromosomal differences between species.

A large-scale whole-genome sequencing analysis reveals highly specific genome editing by both Cas9 and Cpf1 nucleases in rice

This study uses whole-genome sequencing to analyze off-target mutations in Cas9 and Cpf1-edited rice, finding most mutations arise from tissue culture rather than nuclease activity.