Multiplexed Sequential Imaging in Living Cells with Orthogonal Fluorogenic RNA Aptamer/Dye Pairs
R. Zheng, R. Wu, Y. Liu, Z. Sun, Y. Bagheri, Z. Xue, L. Mi, Q. Tian, R. Pho, S. Siddiqui, K. Ren, M. You
This paper introduces seqFRIES, a method for multiplexed live-cell imaging using orthogonal RNA aptamer/dye pairs, achieving four-color imaging in ~20 minutes.
bioRxiv · Apr 20, 2023Bioengineering