Y. Tsuji

Assessing Target of Rapamycin (TOR) activity in the diatom Phaeodactylum tricornutum using commercially available materials

This study develops a Phos-tag-based method to assess TOR kinase activity in diatoms and other algae using commercially available materials, showing dose-dependent and nitrogen-deficient responses.

Periplasmic carbonic anhydrase CAH1 contributes to high inorganic carbon affinity in Chlamydomonas reinhardtii

The study identifies CAH1, a periplasmic carbonic anhydrase in Chlamydomonas reinhardtii, as essential for maintaining high inorganic carbon affinity under CO2-limiting conditions.

Diatom pyrenoids are encased in a protein shell that enables efficient CO2 fixation

This study identifies a protein shell surrounding diatom pyrenoids that enhances CO2 fixation efficiency and reveals its structural and functional role through in vivo and in vitro analyses.

Low-CO2 inducible bestrophins in diatom thylakoid membranes sustain high photosynthetic efficacy at distant locations from the pyrenoid

This study identifies PtBST1 in diatoms as a bestrophin that facilitates HCO3- transport at the stroma thylakoid membrane, enhancing CO2 concentrating mechanism and modulating photoprotection.

Topologically frustrated dynamics in an uncharged Tetra-PEG gel

The paper investigates non-diffusive topologically frustrated dynamics in an uncharged gel matrix and finds a stretched exponential dynamics with exponent around 1/3.

Nuclear activation in dual-durotaxing cells on a matrix with cell-scale stiffness-heterogeneity

This study shows that mesenchymal stem cells undergoing dual-durotaxis on stiff micro-patterned gels experience amplified intracellular stress dynamics and chromatin fluctuations, which enhance cellular vigor and maintain stemness.