The Central Coupler of ClpXP Controls Intersubunit Communication and Couples Intrasubunit Chemical Energy with Force Generation
Robert P. Sosa, Alfredo Jose Florez Ariza, JeongHoon Kim, Alex Tong, Zhenhui Kang, Alicia Li, John Kuriyan, Carlos Bustamante
Q208A mutations in up to four subunits of the ClpX central coupler barely slow unfolded-substrate translocation but raise ATP spent per step, weaken force generation and can abolish GFP unfolding.
bioRxiv · Oct 8, 2026 · PreprintBiophysics✦ In-depth digest