Alex Tong

Q208A mutations in up to four subunits of the ClpX central coupler barely slow unfolded-substrate translocation but raise ATP spent per step, weaken force generation and can abolish GFP unfolding.

This study uses single-molecule force spectroscopy to show that the Rossmann2x2 fold protein folds via a defined pathway of sequential and cooperative folding units.

This study characterizes the effects of three small-molecule inhibitors on MtbRNAP transcription elongation using optical tweezers, revealing distinct inhibition mechanisms and synergistic effects.