Carlos Bustamante

FoxO1 and HNF1α cooperatively remodel nucleosome architecture and mechanical stability to activate transcription

This study shows FoxO1 and HNF1α work together to change nucleosome structure and stability to activate transcription.

The Central Coupler of ClpXP Controls Intersubunit Communication and Couples Intrasubunit Chemical Energy with Force Generation

Q208A mutations in up to four subunits of the ClpX central coupler barely slow unfolded-substrate translocation but raise ATP spent per step, weaken force generation and can abolish GFP unfolding.

The Rossmann2x2 Fold Attains its Native Structure Via a Defined Pathway of Sequential and Cooperative Folding Units

This study uses single-molecule force spectroscopy to show that the Rossmann2x2 fold protein folds via a defined pathway of sequential and cooperative folding units.

A detailed molecular picture of protein folding during active translation

This study uses a hydrogen-deuterium pulse-labeling approach to reveal structural details of nascent polypeptide folding during translation and its kinetic coupling with translation.

Single-Molecule Methods to Investigate Mechanisms of Transcription by RNA Polymerase of Mycobacterium tuberculosis

Apical Localization of RNA Polymerases Modulates Transcription Dynamics and Supercoiling Domains Revealed by Cryo-ET

The paper uses cryo-ET to show RNAP localization at DNA supercoiling apices influences transcription dynamics and supercoiling domains.

A Conserved Metabolic Oxidative Axis Underlies Immune Cell Cryo-vulnerability

This study identifies a conserved metabolic-oxidative pathway causing immune cell cryo-vulnerability and shows that targeted pretreatments can significantly improve post-thaw survival and function.

Spt5's central KOW domains and the Pol II Stalk Collaborate to Regulate Chromatin and 3'-End Processing

This paper shows that Spt5's KOW domains and the Pol II stalk work together in yeast to regulate transcription termination and chromatin structure.

Pleomorphic effects of three small-molecule inhibitors on transcription elongation by Mycobacterium tuberculosis RNA polymerase

This study characterizes the effects of three small-molecule inhibitors on MtbRNAP transcription elongation using optical tweezers, revealing distinct inhibition mechanisms and synergistic effects.