Kumiko Samejima

The paper uses mass spectrometry to analyze histone modifications during mitotic entry, identifying three phosphorylation programs and showing H3 T3 phosphorylation marks heterochromatin, not just centromeres.

This study identifies a bipartite mechanism for condensin II activation in mitosis, showing how M18BP1 relieves autoinhibition and enables DNA loop formation.

In DT40 cells, condensins displace extrusive cohesin, bypass cohesive cohesin and stall against each other while extruding ~1-3 kb/sec, yielding irregular helical chromatids of consecutive loops.

The paper uses cryo-CLEM to visualize and analyze the ultrastructural organization of mitotic chromosomes, identifying non-histone macromolecular densities and surface particle clusters.

The paper shows that common fragile sites (CFSs) have faulty condensin loading leading to chromatin instability and mitotic DNA synthesis during replication stress.

The paper shows mitotic chromosomes form through condensin-mediated disassembly of interphase chromatin and helical winding of loop arrays during prophase and prometaphase.