Steve Reichow

Distinct modes of N-terminal remodeling through a conserved inhibitor pocket in connexin-46/50

This study reveals how two distinct inhibitors affect the conserved N-terminal docking groove of connexin-46/50 gap junctions, leading to different conformational changes and providing structural insights into broad isoform activity.

Calcium induced N-terminal gating and pore collapse in connexin-46/50 gap junctions

This study uses cryo-EM to reveal how calcium binding to connexin-46/50 gap junctions induces structural changes leading to pore collapse and gating.

Multi-ion permeation and dynamic conductance modulation in connexin gap junction channels

This study uses computational simulations to reveal ion permeation mechanisms and dynamic conductance modulation in gap junction channels formed by connexin proteins.

CaMKII T286 autophosphorylation is not propagated at basal Ca2+ levels and is required only for the induction phase of LTP

This study shows that CaMKII T286 autophosphorylation does not propagate at basal Ca2+ levels and is only required during the early phase of LTP induction.

Lipids intercalate and mediate multi-channel assemblies of connexin-46/50 gap junctions

This study determines the structure of multi-channel connexin-46/50 gap junction assemblies, revealing lipid-mediated channel organization and refined single-channel structure.

Reversible lipid mediated pH-gating of connexin-46/50 by cryo-EM

This study uses cryo-EM to reveal how lipid-mediated pH changes gate connexin-46/50 gap junctions, showing reversible structural transitions under acidic conditions.

Mechanism of small heat shock protein client sequestration and induced polydispersity

This study presents cryo-EM structures of sHSPs in apo and client-bound states, revealing mechanisms of client sequestration and polydispersity in sHSP complexes.

Dynamic fibrillar assembly of αB-crystallin induced by perturbation of the conserved NT-IXI motif resolved by cryo-EM.

The paper investigates the structural dynamics of αB-crystallin by mutating the NT-IXI motif, revealing how this affects fibrillar assembly and chaperone function using cryo-EM.

Conserved and divergent features of neuronal CaMKII holoenzyme structure, function, and high-order assembly

This study compares the structure, function, and high-order assembly of neuronal CaMKII and β-isoforms using single-particle EM and biochemical methods, revealing differences in their extended states and clustering behavior.