Lorna McLaren

This study quantifies RecB dynamics at DNA double-strand breaks in E. coli, showing long-lived complexes and intrinsic dissociation properties, and uses RecB binding to estimate damage formation rates.

This study uses single-molecule imaging in E. coli to show that RecB facilitates efficient DNA repair without activating the SOS response, while a modified RecB leads to less efficient repair and high SOS induction.

The paper shows Hfq regulates RecB protein levels in E. coli by reducing translation of recB mRNA, maintaining optimal RecB abundance.

The paper quantifies very low-abundant proteins in bacteria using HaloTag and epifluorescence microscopy, achieving ≥80% detection efficiency.