Achillefs N. Kapanidis

This paper introduces SPIN-Seq, a method that links DNA sequence with functional properties at the single-molecule level, revealing sequence-dependent transcription factor interactions and RNA polymerase behavior.

The paper shows that promoters and terminators in bacteria are often coupled, with termination sequences contributing to promoter function, leading to regulatory interference between adjacent transcription units.

The paper reports the cryoEM structure of TraT, an outer membrane protein involved in plasmid exclusion, and suggests it functions by physical interference rather than specific protein interactions.

This study uses high-throughput DNA-PAINT to analyze influenza virus genome packaging, revealing cooperative and flexible segment interactions that guide genome assembly.

The paper introduces tunable fluorogenic DNA probes that enable high-concentration single-molecule fluorescence imaging, overcoming the high concentration barrier and enabling faster and higher-resolution imaging.

DeepTRACE is a machine learning tool that analyzes single-molecule tracks in living cells, uncovering molecular processes and relationships not present in training data.

This study uses single-molecule imaging in E. coli to show that RecB facilitates efficient DNA repair without activating the SOS response, while a modified RecB leads to less efficient repair and high SOS induction.

This study uses single-molecule tracking to reveal NusG's functional allocation, interactions, and spatial organization in E. coli, showing its roles in transcription elongation, anti-termination, and coupling with translation.

The paper uses single-molecule fluorescence resonance energy transfer to study RNA polymerase trigger loop dynamics, showing it closes and opens on the millisecond timescale and functions as a checkpoint for NTP identity and inhibitor target.

This paper reports the cryo-EM structure of Mycobacterium tuberculosis RNAP holoenzyme bound to fidaxomicin, revealing its unique binding site and mechanism of transcription inhibition.