Alessia Lepore

This study quantifies RecB dynamics at DNA double-strand breaks in E. coli, showing long-lived complexes and intrinsic dissociation properties, and uses RecB binding to estimate damage formation rates.

This study uses single-molecule imaging in E. coli to show that RecB facilitates efficient DNA repair without activating the SOS response, while a modified RecB leads to less efficient repair and high SOS induction.

The paper quantifies very low-abundant proteins in bacteria using HaloTag and epifluorescence microscopy, achieving ≥80% detection efficiency.