Ruth E. Hanna

The paper benchmarks SpCas9 variants to improve base editor screens for BRCA1 and BCL2, identifying new insights into their genetic variants.

This paper uses CRISPR-Cas9 base editors in pooled screens to assess the functional impact of human variants, identifying loss-of-function mutations and drug sensitivity/resistance mutations.

This paper optimizes AsCas12a for combinatorial genetic screens in human cells by improving on-target and off-target activity and enabling higher-order multiplexing.

A genome-wide CRISPR screen with SARS-CoV-2 recovers ACE2 and Cathepsin L and nominates SWI/SNF, TGF-beta signalling and HMGB1 as pro-viral; pathway inhibitors blocked virus-induced death.

The paper presents a method to generate isogenic cell pairs and screen them with CRISPR-Cas9 libraries to identify genetic interactions, demonstrating applications for BCL2L1, MCL1, and PARP1.

The paper evaluates and introduces optimized CRISPR libraries for knockout, interference, and activation screens, showing improved performance in gene function interrogation.

The paper identifies PCR amplification as a major source of uncoupling between sgRNAs and their associated barcodes in combinatorial CRISPR screens and proposes strategies to mitigate this issue.