Molecular BiologySmall StructuresPeer-reviewed
Lock, Protect, and Bind: In Vitro Selection of LNA-modified Aptamers Using a Mutant T7 RNA Polymerase
Kevin Neis, Laia Civit, Kathrine Pedersen…·Julián Valero
This paper introduces a method to efficiently synthesize LNA-modified RNAs using a mutant T7 RNA polymerase, demonstrating its utility in SELEX for generating therapeutically relevant aptamers with high affinity and nuclease resistance.
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