Lock, Protect, and Bind: In Vitro Selection of LNA-modified Aptamers Using a Mutant T7 RNA Polymerase
Kevin Neis, Laia Civit, Kathrine Pedersen, Michael Schachtner, Jesper Thagaard Wengel, Ralf Wagner, Steffen Thiel, Jørgen Kjems, Julián Valero
This paper introduces a method to efficiently synthesize LNA-modified RNAs using a mutant T7 RNA polymerase, demonstrating its utility in SELEX for generating therapeutically relevant aptamers with high affinity and nuclease resistance.
Small Structures · Mar 1, 2026Peer-reviewedMolecular Biology