Yun Wang

This paper introduces a microchip-based DNA synthesis system that significantly improves DNA product concentration and simplifies downstream processes for large-scale gene synthesis.

This study constructs synthetic rDNA arrays in budding yeast and shows that reducing copy numbers or dispersing arrays does not harm cell growth or transcriptomes.

The paper constructs a synthetic yeast chromosome and identifies aging regulators through replicative lifespan studies and genome-scale analysis.

The paper constructs a synthetic disomic yeast using SCRaMbLE and a newly synthesized chromosome to study aneuploidy phenotypes, finding that both copy number and specific chromosomal regions affect fitness.

A de novo yeast neochromosome carrying all 275 tRNA genes was built and characterized; it imposes a growth burden, with genome doubling and deletions in haploids possibly reflecting compensation.

The paper compares the transcriptional landscapes of two castor cultivars using full-length transcriptome and short-read RNA sequencing, revealing significant differences in gene expression and alternative splicing.