Junbiao Dai

CHAIR an antigen-resolved antibody-reactome resource for healthy adult immune aging

CHAIR provides an antigen-resolved immune resource for healthy adult aging by integrating PhIP-seq, clinical data, and immune phenotyping across multiple age groups.

DNA-SaM, a robust system for large-scale data storage

DNA-SaM improves DNA data storage by achieving high efficiency, scalability, and safety through advanced encoding and architecture.

High plasticity of ribosomal DNA organization in budding yeast

This study constructs synthetic rDNA arrays in budding yeast and shows that reducing copy numbers or dispersing arrays does not harm cell growth or transcriptomes.

Manipulating the 3D organization of the largest synthetic yeast chromosome

This paper synthesizes the largest eukaryotic yeast chromosome, synIV, and investigates how its 3D structure affects gene regulation and transcription.

Screening and characterization of aging regulators using synthesized yeast chromosome XIII

The paper constructs a synthetic yeast chromosome and identifies aging regulators through replicative lifespan studies and genome-scale analysis.

Dissecting aneuploidy phenotypes by constructing Sc2.0 chromosome VII and SCRaMbLEing synthetic disomic yeast

The paper constructs a synthetic disomic yeast using SCRaMbLE and a newly synthesized chromosome to study aneuploidy phenotypes, finding that both copy number and specific chromosomal regions affect fitness.

Design, Construction, and Functional Characterization of a tRNA Neochromosome in Yeast

A de novo yeast neochromosome carrying all 275 tRNA genes was built and characterized; it imposes a growth burden, with genome doubling and deletions in haploids possibly reflecting compensation.

Large-scale genomic rearrangements boost SCRaMbLE in Saccharomyces cerevisiae

This study demonstrates that genomic rearrangements in yeast can be efficiently induced using SCRaMbLE, leading to diverse genetic changes and phenotypic effects.

Telomere-to-telomere genome of the model plant Physcomitrium patens

The paper presents a telomere-to-telomere genome assembly for Physcomitrium patens, correcting previous incomplete and erroneous regions and providing resources for moss genomics.

Reconstruct a eukaryotic chromosome arm by de novo design and synthesis

This paper designs and synthesizes a functional neochromosome in yeast that replaces a native chromosome arm, demonstrating genome plasticity and synthetic chromosome viability.

Dissecting PCNA function with a systematically designed mutation library in yeast

The paper constructs a PCNA mutation library in yeast to identify functional residues and reveals that the K168A mutant disrupts the PCNA-Rad5p interaction, essential for DNA damage tolerance.

Cell Lysate Microarray for Mapping the Network of Genetic Regulators for Histone Marks

The paper develops a cell lysate microarray (CLICK) to identify upstream regulators of histone marks using yeast knockout and temperature-sensitive mutant strains, revealing new regulators and enzymes involved in histone acylation.