Shuangying Jiang

High plasticity of ribosomal DNA organization in budding yeast

This study constructs synthetic rDNA arrays in budding yeast and shows that reducing copy numbers or dispersing arrays does not harm cell growth or transcriptomes.

Dissecting aneuploidy phenotypes by constructing Sc2.0 chromosome VII and SCRaMbLEing synthetic disomic yeast

The paper constructs a synthetic disomic yeast using SCRaMbLE and a newly synthesized chromosome to study aneuploidy phenotypes, finding that both copy number and specific chromosomal regions affect fitness.

Design, Construction, and Functional Characterization of a tRNA Neochromosome in Yeast

A de novo yeast neochromosome carrying all 275 tRNA genes was built and characterized; it imposes a growth burden, with genome doubling and deletions in haploids possibly reflecting compensation.

Large-scale genomic rearrangements boost SCRaMbLE in Saccharomyces cerevisiae

This study demonstrates that genomic rearrangements in yeast can be efficiently induced using SCRaMbLE, leading to diverse genetic changes and phenotypic effects.

Reconstruct a eukaryotic chromosome arm by de novo design and synthesis

This paper designs and synthesizes a functional neochromosome in yeast that replaces a native chromosome arm, demonstrating genome plasticity and synthetic chromosome viability.

Cell Lysate Microarray for Mapping the Network of Genetic Regulators for Histone Marks

The paper develops a cell lysate microarray (CLICK) to identify upstream regulators of histone marks using yeast knockout and temperature-sensitive mutant strains, revealing new regulators and enzymes involved in histone acylation.