Samuel R. Hinton

The paper develops a synthetic system to screen chimeric DcuS/EnvZ histidine kinases and identifies key residues affecting ligand responsiveness and selectivity.

DropSynth-Gold uses Golden Gate assembly in emulsions to enable longer and more complex gene library synthesis with comparable fidelity to previous methods.

The paper uses broad mutational scanning to analyze antibiotic resistance mechanisms across diverse DHFR homologs, finding that many variants confer resistance to trimethoprim.

The paper introduces Degenerate DropSynth, a method for assembling diverse gene libraries and local mutants, demonstrating scalability and efficiency in protein engineering.