Author

Karl J. Romanowicz

University of OregonORCID 0000-0001-8732-3272Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Organism:bacteria (other)· 1 Research Focus:drug response / drug discovery· 1 Research Focus:protein design· 1 Assay:protein sequence· 1 Assay:proteomics· 1 Research Focus:software / tool· 1 Research Focus:variant effect prediction· 1

Frequent Co-Authors

Calin PlesaiD· 3 shared Samuel R. HintoniD· 3 shared Andrew S. HolstoniD· 1 shared Carmen ResnickiD· 1 shared Lucas LippertiD· 1 shared Natanya K. VillegasiD· 1 shared

Activity by Year

2025-2026 · peak 2/yr

Co-Author Collaboration Network

6 top collaborators · 10 mutual co-author links

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3 shared papersSynthetic Biology

Calin PlesaORCID

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3 papers

Recent·Most cited

Synthetic BiologyACS Synthetic BiologyPeer-reviewedSep 23, 2026

Characterizing Sequence-Function Relationships in Chimeric DcuS/EnvZ Histidine Kinases at Scale

Lucas Lippert, Andrew S. Holston, Samuel R. Hinton…·Calin Plesa

The paper develops a synthetic system to screen chimeric DcuS/EnvZ histidine kinases and identifies key residues affecting ligand responsiveness and selectivity.

DOI 10.1021/acssynbio.6c00448

proteomicsexperimental assay

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Synthetic BiologybioRxivPreprintJun 1, 2026

DropSynth-Gold: Golden Gate Assembly in Emulsions Extends Multiplexed Gene Libraries to Greater Lengths

Karl J. Romanowicz, Samuel R. Hinton, Natanya K. Villegas, Calin Plesa

DropSynth-Gold uses Golden Gate assembly in emulsions to enable longer and more complex gene library synthesis with comparable fidelity to previous methods.

DOI 10.64898/2026.05.29.728538

experimental assaysoftware / tool

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Synthetic BiologyScience AdvancesPeer-reviewedAug 13, 2025

Exploring Antibiotic Resistance in Diverse Homologs of the Dihydrofolate Reductase Protein Family through Broad Mutational Scanning

Karl J. Romanowicz, Carmen Resnick, Samuel R. Hinton, Calin Plesa

The paper uses broad mutational scanning to analyze antibiotic resistance mechanisms across diverse DHFR homologs, finding that many variants confer resistance to trimethoprim.

10 citations

bacteria (other)virus

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