Júlia Domingo

This study modulates gene dosage using CRISPR and examines nonlinear transcriptional responses to dosage changes in three master transcription factors, revealing non-monotonic effects and links to disease genes.

The paper develops a method to define cell-specific transcription factor regulons and demonstrates their utility in analyzing transcriptional dysregulation in cancer.

The paper identifies target genes and pathways of blood trait loci using CRISPR screens and single-cell RNA sequencing, revealing cis and trans regulatory effects of noncoding variants.

The paper shows that promoter architecture influences co-translational regulation of mRNA levels, with Dbp2 mediating reduced regulation in inducible promoters and ORF sequence playing a major role in constitutive genes.