Eleni P. Mimitou

This study compares immune responses to different COVID-19 vaccines in humans, finding distinct interferon programs in myeloid cells based on vaccine type and dose timing.

This study identifies Fun30 as essential for meiotic DNA break resection, showing it works with MRX and Exo1 to generate resection tracts necessary for recombination.

The paper identifies target genes and pathways of blood trait loci using CRISPR screens and single-cell RNA sequencing, revealing cis and trans regulatory effects of noncoding variants.

The paper introduces CaRPool-seq, a method using Cas13 for combinatorial RNA perturbations in single cells, and applies it to study myeloid differentiation regulators in AML.

The paper uses single-cell multi-omics to study how pathogenic mtDNA mutations are subject to purifying selection in immune cells from Pearson syndrome patients.

The paper introduces scCUT&Tag-pro and scChromHMM to analyze chromatin states in single cells, showing their ability to characterize dynamic changes and regulatory priming across immune cell types.

Weighted-nearest neighbor (WNN) analysis in Seurat v4 learns per-cell modality weights to define cell states, enabling a 228-antibody human PBMC CITE-seq reference for RNA-only query mapping.

This study investigates how antibody concentration, staining volume, and cell number affect signal quality in oligo-conjugated antibody single-cell analysis, offering guidelines to reduce background and improve sensitivity.

The paper uses multi-modal single-cell screens to identify regulators of PD-L1 expression and develops a computational framework to improve analysis of perturbation data.

This study characterizes the structure and function of the Spo11 core complex in S. cerevisiae, revealing its monomeric structure and DNA-binding properties related to meiotic DSB formation.

This paper introduces ASAP-seq, a method to simultaneously profile chromatin accessibility, protein levels, and mtDNA in single cells, demonstrating its application in hematopoietic differentiation and T cell perturbations.

The paper introduces ECCITE-seq, a method that simultaneously captures transcriptomes, immune clonotypes, surface markers, sample identity, and CRISPR perturbations from single cells.

The paper introduces S1-seq, a next-generation sequencing assay to study DNA end resection during yeast meiosis, and describes its experimental and computational methods.

The paper maps meiotic resection endpoints genome-wide in yeast, revealing heterogeneity and the role of Exo1 and Tel1 in resection initiation and processivity.