Kuei‐Ho Chen

The paper identifies an operon in MRSA encoding two secreted nucleases that contribute to virulence, showing attenuation in a mouse model when mutated.

This paper evaluates a DIA proteomics approach using three proteases to increase proteome sequence coverage, achieving higher peptide and amino acid detection rates compared to trypsin alone.

The paper develops a proteomics workflow using orthogonal proteases to generate spectral libraries, enabling comprehensive plasma proteome analysis and identifying TB-related proteins.