Benjamin J. Polacco

The paper generates a CRISPRi perturbation cell atlas in human induced pluripotent stem cells, identifies novel regulators of pluripotency and metabolism, and proposes a machine learning framework for predicting perturbation recipes.

This study investigates how RNA editing in cephalopods alters protein function through proteomics and biochemical assays, revealing its impact on protein stability, localization, and interactions.

This paper introduces a scalable proximity proteomics pipeline that improves reproducibility and throughput in biotinylation-based spatial proteomics.

The paper predicts the mechanistic impacts of human protein missense variants on stability, protein-protein interactions, and small-molecule binding using AlphaFold2 structures.

The paper identifies novel protein and RNA interactions of APOBEC3 enzymes, revealing deamination-independent roles in RNA biology and cancer.

The paper constructs a PPI network of 100 high-confidence ASD genes, identifies novel interactions, and uses AlphaFold-Multimer to prioritize variants for functional validation in organoids.

The paper systematically analyzes proteomic and genomic data of SARS-CoV-2 variants to identify how mutations affect viral gene expression, host interactions, and immune evasion.

The paper shows apoE4 increases VASP phosphorylation, disrupting actin interactions and affecting neurite outgrowth in Neuro-2a cells.

The paper identifies novel regulators of mu opioid receptor signaling and trafficking using proximity labeling and proteomics.

This paper evaluates a DIA proteomics approach using three proteases to increase proteome sequence coverage, achieving higher peptide and amino acid detection rates compared to trypsin alone.

Affinity-purification mass spectrometry of 26 SARS-CoV-2 proteins in HEK293T cells yields 332 high-confidence host interactions and 67 druggable human proteins with known compounds.