Evan W. Miller

Live-cell PAINT microscopy of protein targets via fluorogenic exchangeable HaloTag ligands

The paper introduces a method for live-cell super-resolution microscopy using fluorogenic HaloTag ligands and BF dyes, enabling single-molecule localization and diffusivity mapping of proteins.

Ultrafast Frame-Free Imaging of Neural Activity with Event Cameras

This paper introduces a frame-free imaging method using event cameras to capture neural activity with high temporal resolution and reduced data output, demonstrating its ability to resolve single action potentials and network dynamics.

Tension TRAAKer: a chemigenetic fluorescent membrane tension reporter

The paper introduces Tension TRAAKer, a chemigenetic fluorescent membrane tension reporter that visualizes tension dynamics in living cells with high spatiotemporal precision.

Membrane potential and feedback dynamics regulate CatSper-mediated progesterone signaling in human sperm

This study investigates how progesterone regulates membrane potential and Ca2+ signaling in human sperm through CatSper and Slo3 channels, revealing feedback mechanisms that control depolarization and repolarization.

Towards optical measurements of membrane potential values in Bacillus subtilis using fluorescence lifetime

This paper introduces a fluorescence lifetime-based method to measure membrane potential in Bacillus subtilis, achieving single-cell resolution and quantification of MP changes under chemical perturbations.

Robust self-supervised denoising of voltage imaging data using CellMincer

CellMincer is a self-supervised deep learning method for denoising voltage imaging data, achieving significant noise reduction and improved functional phenotype separation.

Controlling the bioelectrical properties of neurons with ferritin-based Magnetogenetics

This study investigates Magnetogenetics' ability to control neuronal bioelectrical properties using ferritin-based FeRIC constructs, showing RF-induced depolarization and hyperpolarization effects.

Cell-surface targeting of fluorophores in Drosophila for rapid neuroanatomy visualization

The paper introduces genetically targeted membrane tethers for rapid in vivo neuronal labeling in Drosophila using fluorescent dyes.

Voltage imaging in Drosophila using a hybrid chemical-genetic rhodamine voltage reporter

This paper develops a hybrid chemical-genetic voltage reporter for voltage imaging in Drosophila, enabling selective neuron labeling and voltage monitoring in intact tissues.

All-optical electrophysiology in hiPSC-derived neurons with synthetic voltage sensors

The paper introduces a synthetic voltage sensor and optogenetic actuator for all-optical electrophysiology in hiPSC-derived neurons, demonstrating its effectiveness in measuring activity across distinct genetic backgrounds.

Optical spike detection and connectivity analysis with a far-red voltage-sensitive fluorophore reveals changes to network connectivity in development and disease

This paper introduces BeRST 1, a far-red voltage-sensitive fluorophore, and OSCA, a workflow for analyzing neuronal activity, showing changes in connectivity during development and disease.

Imaging voltage in complete neuronal networks within patterned microislands reveals preferential wiring of excitatory hippocampal neurons

The paper uses patterned microislands and voltage imaging to map functional connections in rat hippocampal neuronal networks, revealing preferential connectivity between specific cell types.

Kilohertz frame-rate two-photon tomography

This paper introduces a two-photon imaging technique that achieves high-speed, high-resolution imaging of neural activity in mice at over 1 kHz frame rates.