Laure‐Emmanuelle Zaragosi

Cyclin O is necessary for multiciliated cells to enter their differentiation cell cycle variant and allows the massive amplification of centrioles, which serve as basal bodies for cilia nucleation.

This study evaluates how different culture media affect the differentiation and proliferation of human airway epithelial cells in vitro using single-cell RNA sequencing and imaging.

The paper identifies a new cell cycle variant in multiciliated cells that enables centriole amplification without DNA replication or mitosis.

The paper constructs a single-cell atlas of the human healthy airways using RNA sequencing data from 77,969 cells across 35 locations, revealing cell-type-specific gene expression patterns and cellular heterogeneity.