Nadia Amrani

The paper develops fully modified SpyCas9 guide RNAs that maintain or enhance genome editing efficacy in vitro and in vivo.

GS-Preprocess simplifies GUIDE-seq analysis by converting standard Illumina sequencer output into compatible input for off-target profiling, enhancing accessibility and reducing costs.

The paper demonstrates that NmeCas9, a Cas9 ortholog, exhibits high-fidelity genome editing in human cells with minimal off-target effects and multiple PAM compatibility.

This study introduces NmeCas9 as a new in vivo genome editing tool, demonstrating its effectiveness in targeting specific genes in mice using a single recombinant adeno-associated vector.

The paper identifies and characterizes two new anti-CRISPR protein families that inhibit type II-C Cas9 systems, showing potent inhibition of NmeCas9 and cross-species activity.