Han Zhang

The paper develops fully modified SpyCas9 guide RNAs that maintain or enhance genome editing efficacy in vitro and in vivo.

This paper engineers Nme2Cas9 base editors to improve activity and targeting scope, enabling correction of MECP2 mutations with minimal off-target effects.

This study demonstrates the use of protecting oligos to enhance crRNA stability and potency for in vivo genome editing via AAV co-delivery in mice.

The paper demonstrates the feasibility of using synthetic centromeres in maize to transmit chromosomes across generations through a LexA-CENH3 tethering approach.

This study develops a compact adenine base editor (Nme2-ABE) that can be delivered via a single AAV vector to efficiently edit mouse genomic loci and revert disease mutations in a tyrosinemia model.

The paper reports the genome of Trichoplusia ni, identifies small RNA pathways, and establishes genome editing protocols for Hi5 cells.