Xin D. Gao

OptiPrime predicts prime editing outcomes with high accuracy, enabling efficient therapeutic applications in human and mouse cells.

This study develops a compact adenine base editor (Nme2-ABE) that can be delivered via a single AAV vector to efficiently edit mouse genomic loci and revert disease mutations in a tyrosinemia model.

This paper introduces twin prime editing (twinPE) and its combination with site-specific recombinases to enable precise DNA deletion, replacement, integration, and inversion in human cells without double-strand breaks.

The paper demonstrates that NmeCas9, a Cas9 ortholog, exhibits high-fidelity genome editing in human cells with minimal off-target effects and multiple PAM compatibility.

This study identifies co-regulated alternative splicing events by hnRNPM and ESRP1 during EMT, revealing their antagonistic roles and links to cancer survival.

The paper identifies and characterizes two new anti-CRISPR protein families that inhibit type II-C Cas9 systems, showing potent inhibition of NmeCas9 and cross-species activity.

C-BERST uses dCas9-APEX2 to map proteomes near genomic loci, identifying telomere and centromere-associated proteins.