Aamir Mir

The paper develops fully modified SpyCas9 guide RNAs that maintain or enhance genome editing efficacy in vitro and in vivo.

This paper develops single-AAV vectors for precise in vivo genome editing using Nme2Cas9 with HDR templates or multiple sgRNAs, showing efficacy in treating HT-I and MPS-I mouse models.

This study shows that 5' modifications to DNA repair templates enhance precision genome editing efficiency in multiple animal models and human cells.

This paper demonstrates that circular ssDNA donors improve HDR efficiency and reduce off-target integration compared to linear ssDNA donors in mammalian cells.

The paper demonstrates that NmeCas9, a Cas9 ortholog, exhibits high-fidelity genome editing in human cells with minimal off-target effects and multiple PAM compatibility.

This study introduces NmeCas9 as a new in vivo genome editing tool, demonstrating its effectiveness in targeting specific genes in mice using a single recombinant adeno-associated vector.

The paper identifies and characterizes two new anti-CRISPR protein families that inhibit type II-C Cas9 systems, showing potent inhibition of NmeCas9 and cross-species activity.

C-BERST uses dCas9-APEX2 to map proteomes near genomic loci, identifying telomere and centromere-associated proteins.