Author

James J. Pai

Arc Research InstituteORCID 0000-0001-7247-5142Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Assay:DNA methylation· 1 Research Focus:gene regulatory network inference· 1 Research Focus:genome assembly· 1 Organism:human· 1 Assay:whole-genome sequencing· 1

Frequent Co-Authors

Hiroshi NishimasuiD· 2 shared Masahiro HiraizumiiD· 2 shared Matthew G. DurrantiD· 2 shared Nicholas Thomas PerryiD· 2 shared Patrick D. HsuiD· 2 shared Silvana KonermanniD· 2 shared Aditya R. JangidiD· 1 shared Alison FantoniD· 1 shared

Activity by Year

2024-2025 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 41 mutual co-author links

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2 shared papersBioengineering

Hiroshi NishimasuORCID

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2 papers

Recent·Most cited

BioengineeringSciencePeer-reviewedSep 25, 2025

Megabase-scale human genome rearrangement with programmable bridge recombinases

Nicholas Thomas Perry, Liam J. Bartie, Dhruva Katrekar…·Patrick D. Hsu

This paper describes the engineering of IS622 bridge recombinases to enable efficient and specific DNA rearrangements in the human genome, including insertion, inversion, and excision of up to 0.93 megabases.

41 citationstop 1%

humanwhole-genome sequencing

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GeneticsbioRxivPreprintJan 24, 2024

Bridge RNAs direct modular and programmable recombination of target and donor DNA

Matthew G. Durrant, Nicholas Thomas Perry, James J. Pai…·Patrick D. Hsu

The paper demonstrates that IS110 insertion sequences use a structured RNA to direct sequence-specific DNA recombination, enabling modular genome editing.

7 citations

DNA methylationexperimental assay

𝕏 PostBluesky