Masahiro Hiraizumi

Coordinated RNA- and protein-templated synthesis ofdouble-stranded DNA by a dual reverse transcriptase immune system

The paper shows how two reverse transcriptases with different templating strategies work together to produce complementary DNA for antiviral defense.

Structural mechanism of the type IX Retron-Kva2 anti-phage defense system

This study reveals the structural mechanism of the type IX Retron-Kva2 anti-phage defense system in Klebsiella variicola, showing how it cleaves host RNAs to inhibit phage infection.

Structure-guided development of a potent human B0AT1 inhibitor effective in a mouse model of phenylketonuria

This study develops a potent B0AT1 inhibitor effective in a PKU mouse model by targeting an allosteric site.

Megabase-scale human genome rearrangement with programmable bridge recombinases

This paper describes the engineering of IS622 bridge recombinases to enable efficient and specific DNA rearrangements in the human genome, including insertion, inversion, and excision of up to 0.93 megabases.

Rapid protein evolution by few-shot learning with a protein language model

This paper introduces EVOLVEpro, a few-shot learning framework using protein language models to rapidly improve protein activity with minimal experimental data.

Bridge RNAs direct modular and programmable recombination of target and donor DNA

The paper demonstrates that IS110 insertion sequences use a structured RNA to direct sequence-specific DNA recombination, enabling modular genome editing.

Structural insights into the mechanism of the human SGLT2-MAP17 glucose transporter

The paper determines the structures of the hSGLT2-MAP17 complex with SGLT2 inhibitors, revealing Na+-dependent transport mechanism and biphasic phlorizin binding.

Cryo-EM structures of calcium homeostasis modulator channels in diverse oligomeric assemblies

The paper presents cryo-EM structures of CALHM channels from different species, revealing their ATP conduction and oligomerization mechanisms.