Theresa B. Loveless

This paper introduces peCHYRON, a DNA recorder that sequentially records cellular events in mammalian cells using prime editing, enabling durable, high-information, and temporally resolved molecular recording.

The paper develops a high-throughput assay to profile and engineer TdT variants, identifying variants with altered nucleotide insertion biases that enhance DNA recording capabilities.

This paper introduces CHYRON, a DNA recorder that writes new DNA to track lineage and record cellular stimuli, demonstrating its ability to reconstruct lineage relationships and respond to stress signals.