Charles Sanfiorenzo

Broad-spectrum in situ genetic reprogramming of native microbiomes

The paper develops SPARTA for in situ genetic reprogramming of native microbiomes and introduces ScRibe-seq to identify engineered taxa, demonstrating functionality across diverse environments.

Creation of genomic chimeras via megabase scale genome expansion

This paper introduces ACE, a method to create genomic chimeras by integrating large genomic segments from donor cells into a recipient genome, demonstrating its ability to generate stable chimeric organisms across species barriers.

Rapid in vitro synthesis of DNA templates via Sidewinder for polyadenylated Hantavirus mRNA vaccine candidates

This study develops a rapid DNA template synthesis method called Sidewinder to produce polyadenylated Hantavirus mRNA vaccine candidates in just 2 days.

A universal system for streamlined genome integrations with CRISPR-associated transposases

This paper introduces a CRISPR-associated transposon system for efficient linear DNA integration in E. coli, bypassing traditional limitations through oligonucleotide design and nested PCR.