Alejandro Díaz‐Moscoso

This study optimizes CRISPR-Cas13d for RNA targeting in zebrafish by improving gRNA design, nuclear targeting, and computational prediction, reducing collateral effects.

This study demonstrates that CRISPR-Cas13d efficiently knocks down specific mRNAs in zebrafish, medaka, killifish, and mouse embryos, leading to significant transcript level reduction and embryonic phenotypes.