Ismael Moreno-Sánchez

The paper uses CRISPR-RfxCas13d screening in zebrafish to identify Bckdk as a post-translational regulator of the maternal-to-zygotic transition, showing its role in ZGA and miR-430 processing.

This study optimizes CRISPR-Cas13d for RNA targeting in zebrafish by improving gRNA design, nuclear targeting, and computational prediction, reducing collateral effects.

This study demonstrates the use of SpG and SpRY CRISPR-Cas9 variants in zebrafish and C. elegans for genome editing, showing improved accessibility to previously uneditable sites.

This study demonstrates that CRISPR-Cas13d efficiently knocks down specific mRNAs in zebrafish, medaka, killifish, and mouse embryos, leading to significant transcript level reduction and embryonic phenotypes.