Gopal Kushawah

Spatio-temporally regulated maternal RNA decay factor essential for zebrafish development

The paper identifies Cth1 as a spatio-temporally regulated maternal RNA decay factor essential for zebrafish development, showing its role in promoting deadenylation and decay of maternal mRNAs.

Cth1 is essential for gametogenesis and fertility in zebrafish

This study shows that Cth1 is essential for gametogenesis and fertility in zebrafish, with loss of function causing infertility and gamete abnormalities.

Enhanced RNA-targeting CRISPR-Cas technology in zebrafish

This study optimizes CRISPR-Cas13d for RNA targeting in zebrafish by improving gRNA design, nuclear targeting, and computational prediction, reducing collateral effects.

Critical role of Spatio-Temporally Regulated Maternal RNAs in Zebrafish Embryogenesis

The paper identifies five maternal mRNAs critical for zebrafish embryogenesis and reveals Cth1's role as a spatio-temporal RNA decay factor.

CRISPR-Cas13d induces efficient mRNA knock-down in animal embryos

This study demonstrates that CRISPR-Cas13d efficiently knocks down specific mRNAs in zebrafish, medaka, killifish, and mouse embryos, leading to significant transcript level reduction and embryonic phenotypes.