Miguel A. Moreno-Mateos

This paper characterizes DO1Cas9, a cold-adapted Cas9 nuclease from the deep ocean, showing improved activity under cold conditions and broader organismal applicability.

The paper shows Rab8a-dependent vesicular trafficking transports Wnt8a along cytonemes in zebrafish embryos, affecting Wnt signaling and embryonic patterning.

The paper uses CRISPR-RfxCas13d screening in zebrafish to identify Bckdk as a post-translational regulator of the maternal-to-zygotic transition, showing its role in ZGA and miR-430 processing.

This study optimizes CRISPR-Cas13d for RNA targeting in zebrafish by improving gRNA design, nuclear targeting, and computational prediction, reducing collateral effects.

This study demonstrates the use of SpG and SpRY CRISPR-Cas9 variants in zebrafish and C. elegans for genome editing, showing improved accessibility to previously uneditable sites.

This study demonstrates that CRISPR-Cas13d efficiently knocks down specific mRNAs in zebrafish, medaka, killifish, and mouse embryos, leading to significant transcript level reduction and embryonic phenotypes.

This study shows that Brd4 and P300 regulate zygotic genome activation through histone acetylation in zebrafish embryos.

The study shows the RES complex is essential for zebrafish early development and intron splicing, with specific intronic features associated with its activity.

This study shows that LbCpf1 enables efficient homology-directed repair and temperature-controlled genome editing in zebrafish, improving targeted DNA integration.