Sacha B. Pulsford

This study investigates the molecular basis and evolutionary constraints of organophosphate resistance in dipteran carboxylesterases, revealing sequence context-dependent success of a mutation and trade-offs between affinity and turnover.

This study maps the sequence space of bacterial chitinases to show how domain composition and catalytic core evolve differently based on functional roles.

The paper introduces a sequence analysis pipeline that improves exploration of protein sequence space through hierarchical clustering and alternative homology metrics, demonstrating scalability and utility in analyzing large datasets.

This paper introduces LASE, a method using ancestral sequence reconstruction to train protein language models with higher accuracy and faster computation than large models.

The paper identifies that CsoSCA, a carboxysomal carbonic anhydrase in cyanobacteria, is allosterically activated by RuBP, revealing a regulatory mechanism in CO2 fixation.