MicrobiologybioRxivPreprint
Generation of an optimised Cumate toolkit for tuneable protein expression during in vitro and in vivo studies of Burkholderia cenocepacia
Hamza Tahir, Kristian I. Karlic, Godfrey Mwiti, Nichollas E. Scott
This paper develops an optimized cumate inducible system for precise and tunable protein expression in Burkholderia cenocepacia, both in vitro and in vivo, and demonstrates its utility in studying intracellular replication and O-linked glycosylation.
DOI 10.1101/2025.11.26.690688