Nichollas E. Scott

This study identifies a novel jumbo phage with a tripartite genome architecture and describes its terminal elements' role in infection.

This study identifies LptZ as a critical protein linking surface lipoprotein export to outer membrane vesicle biogenesis in Bacteroides, revealing a novel pathway and expanding the role of the Lpt machinery.

This paper shows that phosphorylation by PknB at T101 promotes WalR dimer formation and strengthens DNA binding, revealing a direct functional link between PknB and WalR.

This study uses CRISPR interference and Nanopore sequencing to investigate RNA modifications in E. coli, revealing their impact on growth and protein pathways.

This study uses N-terminomics to show that CatC inhibition by brensocatib reduces NSP abundance and alters protease-antiprotease network dynamics in HL-60 cells.

This paper introduces an on-bead PAC/SP3 derivatization method for C- and N-terminomics, demonstrating its application in identifying C-terminal peptides and analyzing termini in Jurkat cells.

The paper introduces MassSpectrum Analyzer, a tool for refining proteomic search parameters and re-scoring peptides with modifications, improving detection of highly modified peptides and quantifying fragmentation patterns.

This study quantifies global PPI changes in skeletal muscle insulin resistance using PCP-MS and qXL-MS, revealing dysregulated interactions with chaperones and PDIA6.

This study investigates the role of Dma2 in OMV biogenesis and colonization fitness in Bacteroides thetaiotaomicron, showing that dma2 mutation increases OMV production and affects polysaccharide utilization loci expression, leading to reduced colonization fitness.

The paper identifies and characterizes a functional LolB ortholog in Bacteroides that rescues E. coli's lethal phenotype and shows its role in lipoprotein transport.

This study compares how two intralysosomal pathogens, C. burnetii and L. mexicana, affect lysosomal proteolytic activity and proteome changes in host cells.

This study shows that intracellular Acinetobacter baumannii can act as a reservoir for recurrent lung infections by evading immune responses and antibiotics through a 'persist and resist' strategy.

This paper develops an optimized cumate inducible system for precise and tunable protein expression in Burkholderia cenocepacia, both in vitro and in vivo, and demonstrates its utility in studying intracellular replication and O-linked glycosylation.

This study investigates the relationship between legumain and cathepsins, showing legumain is required for cathepsin processing and nuclear localization of cathepsin L, with implications for cell proliferation and other nuclear functions.

This study identifies glucose-limiting conditions and a GID/CTLH-type E3 ubiquitin ligase as key factors in Toxoplasma differentiation into latent forms.

This study develops pan-specific monoclonal antibodies to detect pseudaminic acids in bacteria, enabling deeper glycoproteomic analysis and improved phagocytosis of multidrug-resistant strains.

This study shows that ogcA and ogcX are conditionally essential for Burkholderia cenocepacia survival, as their loss disrupts O-linked glycan biosynthesis and causes membrane integrity issues.

This paper shows diclofenac sensitizes colistin-resistant A. baumannii to colistin in vitro and in a mouse model, with reduced drug concentrations needed in vivo.

This paper identifies and characterizes Lnb, a lipoprotein N-acyltransferase in Bacteroides, showing its role in lipoprotein biosynthesis and its impact on bacterial growth and polysaccharide degradation.

This study uses cryo-ET to reveal intercellular nanotubes between a DPANN archaeon and its host, providing insights into host-DPANN communication and proteomic changes during symbiosis.

This study develops a heptavalent O-antigen vaccine against Klebsiella pneumoniae, showing varying efficacy against different isolates.

This study shows that O-glycosylation of MIC2 by TgPOFUT2 is not essential for Toxoplasma gondii tachyzoite infectivity and plaque formation.

This study maps protein-protein interactions across seven mouse tissues, revealing over 27,000 unique interactions and showing rewiring of interactions that is not predicted by gene expression.

PrInCE is a bioinformatics pipeline that predicts protein interactomes from co-elution data, offering faster and more accurate results than existing tools.