Hamza Tahir

This paper develops an optimized cumate inducible system for precise and tunable protein expression in Burkholderia cenocepacia, both in vitro and in vivo, and demonstrates its utility in studying intracellular replication and O-linked glycosylation.

This study shows that ogcA and ogcX are conditionally essential for Burkholderia cenocepacia survival, as their loss disrupts O-linked glycan biosynthesis and causes membrane integrity issues.