Kristian I. Karlic

This paper introduces an on-bead PAC/SP3 derivatization method for C- and N-terminomics, demonstrating its application in identifying C-terminal peptides and analyzing termini in Jurkat cells.

The paper introduces MassSpectrum Analyzer, a tool for refining proteomic search parameters and re-scoring peptides with modifications, improving detection of highly modified peptides and quantifying fragmentation patterns.

This paper develops an optimized cumate inducible system for precise and tunable protein expression in Burkholderia cenocepacia, both in vitro and in vivo, and demonstrates its utility in studying intracellular replication and O-linked glycosylation.

This study develops pan-specific monoclonal antibodies to detect pseudaminic acids in bacteria, enabling deeper glycoproteomic analysis and improved phagocytosis of multidrug-resistant strains.

This study shows that ogcA and ogcX are conditionally essential for Burkholderia cenocepacia survival, as their loss disrupts O-linked glycan biosynthesis and causes membrane integrity issues.