Cheryl Kim

The paper uses image-guided sorting to distinguish physically interacting T cell-monocyte pairs from coincidental doublets in tuberculosis patients, revealing distinct transcriptional signatures of contact.

Image-based gating on an imaging spectral cytometer resolves 12 high-purity immune doublet types in human blood; in tuberculosis, doublet frequencies are unchanged but tightly contacting T cell-monocyte doublets increase.

The paper introduces a method to study cell-cell interactions at single-cell resolution, revealing unique transcriptomic signatures in T cell-monocyte complexes during TB and dengue infection.