Thomas J. Scriba

Single-cell profiling of physically interacting immune cells using image-guided sorting

The paper uses image-guided sorting to distinguish physically interacting T cell-monocyte pairs from coincidental doublets in tuberculosis patients, revealing distinct transcriptional signatures of contact.

A high-resolution blood immune cell doublet atlas via imaging spectral cytometry

Image-based gating on an imaging spectral cytometer resolves 12 high-purity immune doublet types in human blood; in tuberculosis, doublet frequencies are unchanged but tightly contacting T cell-monocyte doublets increase.

Inflammatory Biomarkers of Asymptomatic and Symptomatic Tuberculosis

This study identifies transcriptomic and proteomic features of asymptomatic tuberculosis, revealing distinct sub-clusters and a new blood signature, but finds weaker diagnostic performance for asymptomatic cases compared to symptomatic ones.

Subunit vaccination enhances protection conferred by prior Mycobacterium tuberculosis exposure

This study shows that subunit vaccination provides better protection than BCG in Mtb-exposed individuals, supported by mouse models and human data.

Conserved T cell receptor usage underpins recognition of CD1c presenting a mycobacterial lipid

The paper identifies a conserved TCR motif recognizing mycobacterial lipids presented by CD1c, showing higher frequencies in TB-endemic regions and providing a cryo-EM structure of the interaction.

Integration of immunomonitoring assays with PET/CT in TB patients identifies on-treatment biomarkers

The paper identifies on-treatment biomarkers in TB patients by integrating immunomonitoring assays with PET/CT scans, showing significant cytokine changes and machine learning-based biomarkers for treatment response.

Enrichment of a CD4-CD8- NK-like cytotoxic Vδ1/3 T cell subset in tuberculosis disease

This study identifies an enriched NK-like cytotoxic Vδ3 T cell subset in TB patients using single-cell RNA sequencing, showing persistent transcriptomic differences post-treatment.

Cross-species blood transcriptional correlates of BCG-mediated protection against tuberculosis include innate and adaptive immune processes

This study identifies cross-species blood transcriptional correlates of BCG-mediated protection against tuberculosis using a systems modeling framework, linking innate and adaptive immune processes to vaccine efficacy in humans and non-human primates.

BCG Vaccination at Birth Shapes the TCR Usage and Functional Profile of MR1T Cells at 9 Weeks of Age

This study examines how BCG vaccination at birth affects MR1T cell TCR usage and function in infants, finding increased type I interferon response and a TCR cluster enriched in vaccinated infants.

A novel method for characterizing cell-cell interactions at single-cell resolution reveals unique signatures in blood T cell-monocyte complexes during infection

The paper introduces a method to study cell-cell interactions at single-cell resolution, revealing unique transcriptomic signatures in T cell-monocyte complexes during TB and dengue infection.

Peptide Driven Identification of TCRs (PDI-TCR) reveals dynamics and phenotypes of CD4 T cells in tuberculosis

PDI-TCR identifies Mtb-specific TCRs and characterizes their phenotypes in TB patients and healthy individuals, revealing distinct dynamics of specific and unspecific T cell responses.

Mtb specific HLA-E restricted T cells are induced during Mtb infection but not after BCG administration in non-human primates and humans

Neutrophil degranulation, NETosis and platelet degranulation pathway genes are co-induced in whole blood up to six months before tuberculosis diagnosis

The paper identifies gene pathways associated with neutrophil and platelet responses that are activated up to six months before TB diagnosis in whole blood.

Multinomial modelling of TB/HIV co-infection yields a robust predictive signature and generates hypotheses about the HIV+TB+ disease state.

The paper develops a 10-gene random forest signature to predict TB and HIV status and distinguishes TB/HIV co-infection from other conditions with high accuracy.