Mark A. Pfuhl

The paper designs and validates a de novo protein binder for the titin M10 domain, demonstrating its utility in fluorescence microscopy without antibodies.

This paper develops a three-color Cascade-FRET system to measure force-dependent interactions between RIAM and vinculin in focal adhesions, revealing force-independent binding and tension-sensing capabilities.

This study determines the structure of the ch-TOG-TACC3 interaction and shows that inhibiting it causes pericentriolar material fragmentation and delayed mitosis.

This study investigates how mutations in POPDC proteins affect their heteromeric interaction and membrane trafficking, finding that complex formation is essential for membrane targeting.