Yuki Kawai‐Harada

This study develops CRISPR-Cas9 engineered HEK293T cells for stable production of surface-engineered extracellular vesicles, showing improved consistency and scalability compared to transient transfection.

The paper develops a method to select tissue-specific proteins that direct extracellular vesicles in vivo, using EV-display libraries and a Markov chain model to predict selection dynamics.

This paper introduces a scalable filtration method to isolate exomeres, showing they are stable, miRNA-rich NVEPs that could improve RNA delivery.

This paper introduces RecombiCraft, a method for DNA library construction in E. coli using SLiCE and liquid culture, which minimizes sequence bias and reduces costs.

This paper introduces a method for isolating surface-engineered extracellular vesicles using TFF-SEC, showing improved purity and efficiency compared to conventional methods.