Gustavo Aguilar

Tag-stabilized fluorescent protein binders for endogenous cell type-specific protein labelling

This paper develops fluorescent protein binders for cell type-specific visualization of endogenous proteins, showing improved signal-to-noise ratio and application in multiple organisms.

In situ mutational screening and CRISPR interference define apterous cis-regulatory inputs during compartment boundary formation

The paper identifies cis-regulatory elements controlling apterous expression during Drosophila wing compartment boundary formation using CRISPR interference and mutational analysis.

Shaping and interpretation of Dpp morphogen gradient by endocytic trafficking

The paper investigates how endocytic trafficking shapes and interprets the Dpp morphogen gradient in Drosophila, showing that Dynamin-mediated internalization shapes the gradient while MVB formation affects its interpretation.

Direct cell-to-cell transport of Hedgehog morphogen is aided by the diffusible carrier Shifted/DmWif1

This study identifies Shf as a lipid-shielding protein that facilitates cell-to-cell transport of Hedgehog morphogen via cytonemes.

In vivo regulation of an endogenously-tagged protein by a light-regulated kinase

This paper develops a light-inducible method to phosphorylate endogenous Myosin and shows it disrupts cytoskeletal dynamics in Drosophila embryos.

Heterodimerization-dependent secretion of BMPs in Drosophila

The paper investigates the formation and function of BMP heterodimers in Drosophila, showing they are essential for signaling and wing development.

In vivo regulation of fluorescent fusion proteins by engineered kinases

The paper develops engineered kinases to specifically phosphorylate fluorescent protein fusions in vivo, demonstrating their use in studying actomyosin activation in Drosophila embryos.

In vivo seamless genetic engineering via CRISPR-triggered single-strand annealing

The paper introduces SEED/Harvest, a CRISPR-based method for efficient and controlled knock-in generation in multicellular animals, enabling seamless cassette removal and spatio-temporal protein labeling.

Glypicans specifically regulate Hedgehog signaling through their interaction with Ihog in cytonemes

This study investigates how glypicans regulate Hedgehog signaling by interacting with Ihog in cytonemes in Drosophila.